Genotyping of strawberry varieties for loci of resistance to anthracnose (Colletotrichum spp.)
Keywords:
Fragaria × ananassa, variety, resistance, Colletotrichum acutatum, Colletotrichum gloeosporioides, molecular markers, marker-assisted selectionAbstract
Molecular marking of 54 strawberry varieties (Fragaria × ananassa) for resistance loci to Colletotrichum acutatum (FaRca1, Rca2) and C. gloeosporioides(FaRCg1) was carried out. At least one of the studied resistance loci is present in 73.7 % of the analyzed strawberry varieties. Resistance alleles to C. acutatum of the pathogenicity group 1 (FaRca1) and the pathogenicity group 2 (Rca2) were detected in 40.3 % and 12.3 % of the analyzed varieties. All identified varieties – carriers of the FaRca1 and Rca2 alleles have a heterozygous genotype. The FaRCg1 resistance locus to C. gloeosporioides is present in 40.3 % of samples. A statistically significant predominance of the FaRCg1 locus was noted in Russian strawberry varieties. The identified varieties – carriers of the FaRCg1 allele can have a homozygous or heterozygous genotype. Two loci out of three in three variants of the combination of resistance alleles (FaRca1+FaRCg1, FaRca1+Rca2, FaRCg1+Rca2) were detected in 15.8 % of samples, and in most cases (77.8 %) this is a combination of the FaRca1 and FaRCg1 loci. Among Russian strawberry samples, this allelic combination has been identified in the varieties Zenit, Neznakomka, Urozhaynaya TSGL, and Flora; among foreign samples – in the varieties Korona, Salsa, and Vicoda. The combinations FaRca1+Rca2 and FaRCg1+Rca2 characterize the varieties Aprica and Sudarushka, respectively. Three resistance alleles were identified in the strawberry variety Borovitskaya (1.7 % of the total number of samples). The indicated strawberry varieties are promising complex genetic sources of alleles of resistance to anthracnose caused by C. acutatum and C. gloeosporioides.
