THE INFLUENCE OF GLYPHOSATE IN COMBINATION WITH ANTIBIOTICS ON THE MICROBIAL COMMUNITY OF BROILER’S CECUM ACCORDING TO WHOLE GENOME SEQUENCING

Abstract

To evaluate the effects of antibiotics and glyphosate, an experiment was conducted on 260 broilers. Four experimental groups were formed: I - control group, which received the basic diet (BD), II experimental group - BD with the addition of glyphosate; III experimental - OR with the addition of glyphosate and veterinary antibiotics enrofloxacin and colistin methanesulfonate; IV experimental - OR with the addition of glyphosate and ammonium maduramicin. Glyphosate and antibiotics combined with a herbicide altered the microbial community in broiler’s cecum. Genome-wide analysis made it possible to estimate the increase in the proportion of eukaryotic microorganisms and viruses under the influence of antibiotics. In the group of prokaryotic microorganisms, under the influence of glyphosate and antibiotics, significant changes were noted associated with a decrease in the proportion of bacteria, such as Faecalibacterium sp., Lawsonibacter sp., Lachnoclostridium sp. and Subdoligranulum sp., capable of producing butyrate and other short-chain fatty acids. These results indicate the negative impact of glyphosate in combination with antibiotics on the health and productivity of poultry, since these acids have a wide range of positive properties, for example, bacteriostatic, anti-inflammatory and proliferative effects.